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产品中心 分子酶 mRNA疫苗和siRNA相关药物

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RNase H

Ribonuclease HI、RNase HI、Ribonuclease H、RNase H

价格 ￥420.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070045
规格
数量
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产品介绍 评论(0)

产品规格

  • 分子别名

    Ribonuclease HI、RNase HI、Ribonuclease H、RNase H

  • 分子量

    19 kD

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    5U/μl

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C）

  • 溶解方法

    /

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    [1] Chan S H , Whipple J M , Dai N ,et al.RNase H-based analysis of synthetic mRNA 5' cap incorporation[J].RNA (New York, N.Y.), 2022, 28(8):1144-1155.

    [2] Ilina T V , Brosenitsch T , Sluis-Cremer N ,et al.Retroviral RNase H: Structure, mechanism, and inhibition[J]. 2021.

  • 稀释度

背景介绍

  • Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA).

产品组分

  • Storage Solution: 5U/μl RNase H、50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C） 10*Reaction Buffer: 500 mM Tris-HCl、750 mM KCl、30 mM MgCl2、100mM DTT(pH 8.3 @ 25°C)

操作步骤

  • Set-up a typical reaction as follows 1）Add the following components in sequence 2）Incubate at 37°C for 20 minutes This reaction can be scaled up according to experimental needs.

注意事项

  • 1、Both metal ion chelating agent and sulfhydryl sealer can inhibit RNase H activity 2、Heating at 65℃ can be inactivated for 10min

酶活定义
  • One unit is the amount of enzyme required to hydrolyze 1 nmol nucleotide from a 20 pmol fluorescent-labeled 50 bp RNA-DNA hybridization chain at 37℃ for 20 minutes in a 50 µl reaction system.

  • 生物活性

    • Marker（100 bp ~ 2000 bp）:1 ul
      Lane 1、DNA:RNA duplex Substrate
      Lane 2、DNA:RNA duplex Substrate + Competitive product RNase H（5 U/ul）1 ul
      Lane 3、DNA:RNA duplex Substrate + UA RNase H（5 U/ul）1 ul
      Lane 4、DNA:RNA duplex Substrate + UA RNase H（5 U/ul）2 ul
      Lane 5、DNA:RNA duplex Substrate + UA RNase H（5 U/ul）3 ul

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

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