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HRV 3C Protease

Protease 3C、PreScission Protease

价格 ￥200.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070044
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产品介绍

产品介绍 评论(0)

产品规格

  • 分子别名

    Protease 3C、PreScission Protease

  • 表达宿主

    E.coli

  • 分子量

    21 kD (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    1U/μl

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v/v) glycerol，（pH 8.0 @ 25℃）

  • 溶解方法

    /

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    [1] Knott J A , Orr D C , Montgomery D S ,et al.The expression and purification of human rhinovirus protease 3C.[J].European Journal of Biochemistry, 1989, 182(3):547-55.

    [2] Xu H , Wang Q , Zhang Z ,et al.A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease[J].Enzyme and Microbial Technology, 2019, 123:15-20.

背景介绍

  • Recombinant human rhinovirus(HRV)3C protease is widely used in the purification and productions of proteins due to its good stability，high specificity，and enzymatic activity．3C protease is one of the non—structural proteins of HRV，and plays important roles in the life cycle of HRV．This protease specifically recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, and cleaves the peptide bond between Gln and Gly.

产品组分

  • Storage Solution: 1U/μl 3C Protease50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v/v) glycerol（pH 8.0 @ 25℃） 10*Reaction Buffer: 500mM Tris-HCl，1.5M NaCl，10mM EDTA，10mM DTT，（pH 7.5@ 25℃）

操作步骤

  • 1. Combine 100 μg of fusion protein, 10 μl of 10*Reaction Bufferr and H20 (if necessary) to make a 100 μl total reaction volume. 2. Add 1 µl HRV 3C Protease 3. Incubate reaction at 5°C for 16 hour.

注意事项

  • Please avoid repeated freeze-thaw cycles.

酶活定义
  • One unit is defined as the amount of enzyme required to remove > 90% of the GST label from 100 µg of fusion protein at 5°C for16 hour.

  • 生物活性

    • The results of 100μg substrate digestion separated under different quantity of 3C Protease, the reaction was incubated for 16h minutes at 5°C.

  • 电泳

  • 反相高效液相色谱(RP-HPLC)

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