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Murine RNase Inhibitor

Ribonuclease inhibitor

价格 ￥200.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070037
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Rat

  • 分子别名

    Ribonuclease inhibitor

  • 表达宿主

    E.coli

  • 分子量

    50 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    40 U/μL

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 缓冲体系

    20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    [1] Wanhua, Guo, and et al. High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners[J]. Protein Expression & Purification, 2011.
    [2] Chen, C. Z. , and R. Shapiro . "Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A. " Proceedings of the National Academy of Sciences 94.5(1997):p. 1761-1766.

背景介绍

  • Murine RNase inhibitor is expressed in soluble form in E. coli and capable of inhibiting a wide range of RNases (RNase A, B, C). Murine RNase inhibitor was tested by RT-PCR and RT-qPCR, and was compatible with various commercial reverse transcriptase’s and various DNA polymerases. Compared with human RNase inhibitor, murine RNase inhibitor does not contain two cysteines that are very sensitive to oxidation, so it has higher antioxidant activity and is more suitable for high DTT sensitive experiments (such as qPCR).

产品组分

  • Storage Solution: 40 U/µL Murine RNase Inhibitor, 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)

操作步骤

  • Add inhibitor to achieve final concentration of 1 U/μL in your reaction.
    During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination.

注意事项

  • Please avoid repeated freeze-thaw cycles.

酶活定义
  • One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.

  • 生物活性

    • In the experimental design, 4 μg RNA was incubated with 0 or 2.5 ng RNase A and 1, 0.5, 0.25, 0.125 or 0 μL RNase Inhibitor in 20 μL reaction system, incubated for 15 minutes at 37°C. The reaction was analyzed by 1% agarose gel electrophoresis. The figure is the effect of RNase Inhibitor on RNase A enzyme activity.
      Lane 1 Negative Control (Only negative controls with no RNase A were added);
      Lane 2 UA-Murine RNase Inhibitor 0 μL;
      Lane 3 UA-Murine RNase Inhibitor 0.125 μL;
      Lane 4 UA-Murine RNase Inhibitor 0.25 μL;
      Lane 5 UA-Murine RNase Inhibitor 0.5 μL;
      Lane 6 UA-Murine RNase Inhibitor 1 μL;

  • 电泳

    • 1 μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

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