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物种
Bacillus stearothermophilus分子别名
BsaⅠ 限制性内切酶表达宿主
E.coli分子量
64.9kDa (Reducing)
纯度
>95% by SDS-PAGE活性
20 U/μL标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
10 mM Tris-HCl、300 mM NaCl、1 mM DTT、0.1 mM EDTA、500 µg/ml BSA、50% Glycerol （pH 7.4 @ 25°C）储存条件
Store at -25 ~ -15℃ for 2 years
文献引用
[1] Zhu Z, Samuelson J C, Zhou J, et al.Engineering Strand-specific DNA Nicking Enzymes from the Type IIS Restriction Endonucleases BsaI, BsmBI, and BsmAI[J].Journal of Molecular Biology, 2004, 337(3):573-583.
[2] Lee J H, Won H J, Oh E S,etal. Golden Gate Cloning-Compatible DNA Replicon/2A-Mediated Polycistronic Vectors for Plants[J]. Frontiers in Plant Science, 2020, 11.
BsaI is a Type IIs restriction enzyme that can recognize non-palindromic sequences and cut outside of the recognition sequence. It is commonly used for Golden Gate assembly and enzymatic cleavage of plasmids to prepare linear DNA fragments with poly (A/T/G/C) endings and obtain specific sticky ends.
Recognition site:
5'-GGTCTC(N)1↓-3'
3'-CCAGAG(N)5↑-5'
Storage Solution: 20 U/μl BsaⅠ、10 mM Tris-HCl、300 mM NaCl、1 mM DTT、0.1 mM EDTA、500 µg/ml BSA、50% Glycerol（pH 7.4 @ 25°C）
10*Reaction Buffer：500 mM Potassium Acetate、200 mM Tris-acetate、100 mM Magnesium Acetate、1mg/ml BSA（pH 7.4 @ 25°C）
1. star activity may result from a glycerol concentration of >5%; 2. Please avoid repeated freeze-thaw cycles.
- One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
生物活性
The results of 1μg pUC57 plasmid digestion separated under different quantity of BsaⅠ, The reaction was incubated for 60 minutes at 37°C, and 1% agarose gel was used for electrophoresis analysis after reaction.
M, marker;
Lane 1 1μg pUC57;
Lane 2 1μg pUC57 add 1U BsaⅠ
Lane 3 1μg pUC57 add 2U BsaⅠ
电泳
1μg (R: reducing condition, N: non-reducing condition).
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