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物种
Streptococcus pyogenes分子别名
IdeS、IdeS Protease、Immunoglubulin-degrading enzyme表达宿主
E.coli分子量
38 kDa (Reducing)
纯度
＞95% by SDS-PAGE标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS pH 6.6储存条件
·12 months from date of receipt, -20 to -70 °C as supplied.
· It can be stored at 4-8℃ for one week after dissolution, under sterile conditions
· Please avoid repeated freeze-thaw cycles.
文献引用
[2] Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615.
IdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcus pyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs
- One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.
生物活性
The results of 5μg rabbit IgG digestion separated under different quantity of IdeS Protease(Tag Free), The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).
M, marker;
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeS Protease (Tag Free);
lane 3 rabbit IgG add 10units IdeS Protease (Tag Free);
lane 4 rabbit IgG add 16.7 units IdeS Protease (Tag Free);
lane 5 rabbit IgG add 50 units IdeS Protease (Tag Free);
电泳
2μg (R: reducing condition, N: non-reducing condition).
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