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物种
Photinus pyralis表达宿主
E.coli分子量
61.7kDa
纯度
>95% by SDS-PAGE活性
3.1×10^7 CPS/mg标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
20 mM Tris (pH7.4), 1 mM EDTA, 1 mM DTT, 50% glycerol
储存条件
12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
文献引用
1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81.
2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287.
Luciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. In our work, the luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, some biological applications are limited by the low stability of the luciferase, so we combined amino acid mutations to enhance the enzyme’s thermostability and was eventually named Luciferase Ⅱ.
- One unit of Luciferase is defined as the chemiluminescence module of PerkinElmer VICTROR Nivo was used to record the luminescence reading output within 5 minutes, namely the luminescence quantity CPS.
电泳
M: marker ; Luciferase Ⅱ ，2μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%
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