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物种
Photinus pyralis分子别名
N.A表达宿主
E.coli分子量
62kDa
纯度
>95% by SDS-PAGE活性
3.5×10^8CPS/mg标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
25 mM Tris-acetate (pH7.8),1 mM EDTA, 1 mM DTT, 50% glycerol, 0.2 M ammonium sulfate
溶解方法
N.A
储存条件
12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
文献引用
1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81.
2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287.
Luciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. The Luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, wild-luciferase activity is inhibited by sodium chloride, Therefore, the amino acid mutation we performed improved the inhibition of sodium chloride and was eventually named Luciferase Ⅰ.
- One unit of Luciferase is defined as the chemiluminescence module of PerkinElmer VICTROR Nivo was used to record the luminescence reading output within 5 minutes, namely the luminescence quantity CPS.
电泳
M: marker ; Complement Luciferase Ⅰ，2μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.
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