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产品中心 酶制剂 抗体及糖基化切割

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IdeZ Protease

IgG endopeptidase

价格 ￥2,310.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070028
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Streptococcus pyogenes

  • 分子别名

    IgG endopeptidase

  • 表达宿主

    E.coli

  • 分子量

    36kDa

  • 纯度

    >95% by SDS-PAGE

  • 活性

    10U/μL

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5

  • 溶解方法

    N.A

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied;

    1 week, 2 to 8 °C under sterile conditions;

    Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26.

    2.Cheng-Wei H, Jie P, Yu-Qing O, et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J]. Chinese Journal of Preventive Veterinary Medicine, 2018.

背景介绍

  • IdeZ Protease secreted by the Streptococcus equi subspecies zooepidemicus. This product is an engineered recombinant protease over expressed in Escherichia coli. It can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and produce F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.

操作步骤

  • 1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.

    2. Add IdeZ Protease to the reaction system:

    • Add 1 unit of IdeZ Protease per 1µg of IgG to be digested.

    • For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg of IgG.

    3. Incubate sample at 37°C for 30–60 minutes


酶活定义
  • One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C

  • 生物活性

    • Results of 5μg rabbit IgG digestion separated under different quantity of IdeZ protease, The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate)

      M, marker:

      lane 1 rabbit IgG;

      lane 2 rabbit IgG add 5 units IdeZ;

      lane 3 rabbit IgG add 10 units IdeZ;

      lane 4 rabbit IgG add 33units IdeZ;

      lane 5 rabbit IgG add 50 units IdeZ

  • 电泳

    • M: marker; Complement IdeZ protease, 2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%

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