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物种
Streptococcus pyogenes分子别名
IdeS表达宿主
E.coli分子量
37.7kDa
纯度
>95% by SDS-PAGE活性
40U/μl标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
PBS pH 6.6, 50% glycerol溶解方法
N.A
储存条件
12 months from date of receipt, -20 to -70 °C as supplied;
1 week, 2 to 8 °C under sterile conditions;
Please avoid repeated freeze-thaw cycles.文献引用
1.Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterization of the streptococcal endopeptidase, IdeS, reveals that it is a cysteine protease with strict specificity for IgG cleavage due to exosite binding.[J].Biochemistry, 2004, 43(49):15540-15549.
2.Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615.
IdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcuspyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.
- One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.
生物活性
- Results of 5μg rabbit IgG digestion separated under different quantity of IdeS, The reaction was incubated for8 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).
M, marker;
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeS;
lane 3 rabbit IgG add 6.25 units IdeS;
lane 4 rabbit IgG add 8.3 units IdeS;
lane 5 rabbit IgG add 12.5 units IdeS;
lane 6 rabbit IgG add 25 units IdeS
电泳
M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing condition . The purity is greater than 95%.
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