分享产品介绍 评论(0)
物种
Human抗原名称
ELOB/ELOC/VHL分子别名
Elongin B & Elongin C & VHL, ELOB & ELOC & VHL Complex, Elongin B & Elongin C & VHL ComplexAccession
Q15370, Q15369, P40337表达序列
ELOB flag tag, Human, Asp2-Gln118, N-flag ELOC flag tag, Human, Asp2-Cys112, N-flag VHL His tag, Human, Pro2-Asp213, N-His
表达宿主
Baculovirus-InsectCells分子量
14 kDa (ElOB) and 13.3 kDa (ElOC) and 25.9 kDa (VHL)
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated性状
Liquid缓冲体系
40mM Tris, 200mM NaCl, 2.2mM KCl, 20% glycerol, 3mM DTT, pH8.0
溶解方法
N.A
储存条件
12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
文献引用
1.Cell Chem Biol. 2023 Jul 20;30(7):766-779.e11. Epub 2023 Jun 23.
2.bioRxiv. 2023 May 24;2023.05.22.541439. PreprintOkumura F., et al. (2012) Front. Oncol.
3.Mod Pathol. 2023 Apr 23;36(8):100194. Online ahead of print.
ELOB (elongin B) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. ELOB and ELOC(elongin C) form a heterodimer that serves as the regulatory subunit for the Elongin complex-a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. The VHL(von Hippel-Lindau disease tumor suppressor) binds to ELOB and ELOC and thereby inhibits transcription elongation.
电泳
4μg (R: reducing condition).
SPR
VHL/Elongin-C/Elongin-B Complex Protein, Human (Cat. No. UA070023) on CM5 Chip can bind MZ1 with an affinity constant of 1.30 μM as determined in a SPR assay .
评论(0)