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Hyaluronidase PH20/SPAM1, Human (GMP)

Sperm adhesion molecule 1,SPAM1,Vorhyaluronidase alfa,rHuPH20

价格 ￥7,200.00 （ 1-2周）
货号 UA070006-GMP
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Sperm adhesion molecule 1, SPAM1, Vorhyaluronidase alfa, rHuPH20

  • Accession

    P38567

  • 表达序列

    Leu36-Tyr482

  • 表达宿主

    CHO

  • 宿主蛋白残留

    ＜100ppm

  • 宿主DNA残留

    ＜100ppb

  • 支原体

    阴性

  • 无菌

    符合

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Liquid

  • 缓冲体系

    150mM sodium chloride, 10mM dibasic sodium phosphate, 15mM albumin human, 10mM L-methionine, 0.02% polysorbate 80, pH6.2±0.1

  • 储存条件

    · 12 months from date of receipt, liquid stored at -20 to -80℃.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Active Expression of Human Hyaluronidase PH20 and Characterization of Its Hydrolysis Pattern. Bo Pang; Jing He; Weijiao Zhang; Hao Huang; Yang Wang; Miao Wang; Guocheng Du; Zhen Kang; Front Bioeng Biotechnol. 2022 May 13:10:885888;
    A recombinant human enzyme for enhanced interstitial transport of therapeutics. L.H. Bookbinder a , A. Hofer a , M.F. Haller a , M.L. Zepeda b , G.-A. Keller a , J.E. Lim a ,T.S. Edgington c , H.M. Shepard d , J.S. Patton e , G.I. Frost a, Journal of Controlled Release 114 (2006) 230–241.

背景介绍

  • Hyaluronidases are a group of glycosidases catalyzing the degradation of hyaluronic acid (HA). There are six known types (hyaluronidase 1–4, PH-20, and HYALP1). Human hyaluronidase is present both in organs (testis, spleen, skin, eyes, liver, kidneys, uterus, and placenta) and body fluids (tears, blood, and semen). Testicular PH20 hyaluronidase is found on the surface of human sperm and inner acrosomal membrane and degrades hyaluronic acid in the ovum during fertilization. Hyaluronidase PH20 is known as sperm adhesion molecule 1 (SPAM1) and random hydrolysis of (1, 4)-linkages between N-acetyl- beta-D-glucosamine and D-glucuronate residues in hyaluronic acid (HA). PH20/SPAM1 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. PH20/SPAM1 protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

酶活定义
  • The Hyaluronidase PH20, Human (GMP) has been assayed by turbidimetric, according to Pharmacopoeia of the People's Republic of China.

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