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产品中心 酶制剂 融合标签切割酶

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Enterokinase, Bovine

rBoEnterokinase,Enteropeptidase,ENTK,PRSS7

价格 ￥1,100.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070001
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Bovine

  • 分子别名

    rBoEnterokinase; Enteropeptidase; ENTK; PRSS7,

  • 表达宿主

    E.coli

  • 分子量

    28 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE and HPLC

  • 活性

    5 U/μL

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 , 50% Glycerol(pH 7.4 @ 25°C)

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    1.Yong S Z, Yong C J, Yuan C X, et al. Secretory Expression, Purification and Characterization of Bovine Enterokinase Light Chain[J]. Journal of Nanjing University (Natural Sciences), 2004.
    2.Haidong, Tan, and, et al. Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli[J]. Protein Expression & Purification, 2007.

背景介绍

  • Enterokinase (enteropeptidase, EC 3.4.4.8) occupies a key position in the utilization of dietary proteins. The enzyme initiates intraluminar digestion of proteins by the proteolytic conversion of trypsinogen to trypsin, which in turn activates the other pancreatic zymogens (Kunitz, 1939a,b; Hadorn et al., 1969). The proteolytic attack of enterokinase is directed exclusively toward the Lys6-Ile7 peptide bond of trypsinogen leaving all other lysine and arginine bonds in the molecule unaffected (Maroux et al., 1971). The resultant cleavage produces the simultaneous release of active trypsin and of the
    amino-terminal hexapeptide Val-(Asp)d-Lys (Rovery et al., 1953; Davie and Neurath, 1955). This unique specificity exhibited by enterokinase is of interest as it relates to both the molecular basis of substrate recognition and the control of the digestive process.

产品组分

  • 5 U/μL Enterokinase, Bovine in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 , 50% Glycerol(pH 7.4 @ 25°C)

操作步骤

  • Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
    1. Combine 500 ug of sample with reaction buffer
    * Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)
    2. Add 1 U of Enterokinase light chain
    3. Incubate at 25°C for 16 hours

注意事项

  • 1. Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50 mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.

酶活定义
  • One unit is defined as the amount of enzyme required to cleave 500 µg of substrate to 95% completion in 16 hours at 25°C.

  • 生物活性

    • The results of 100μg substrate digestion separated under different quantity of UA070001, the reaction was incubated for 16 h at 25°C.
      M marker
      Lane 1 Only substrate
      Lane 2 Substrate/ UA070001 = 4000/1 (1U)
      Lane 3 Substrate/ UA070001 = 2000/1
      Lane 4 Substrate/ UA070001 = 1000/1

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • 98%

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