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物种
Human分子别名
MSP1D1E3浓度
1.8mg/ml by Nanodrop表达序列
Target(266 amino acid) with N terminal 6x His and Factor Xa site
表达宿主
E.coli分子量
32.68 kDa
纯度
> 90% as determined by SDS-PAGE & SEC-HPLC.标记
Unconjugated标签
His Tag缓冲体系
40 mM Tris, ph8.0, 300 mM NaCl.
储存条件
·6 months from date of receipt, -60 to -80 °C as supplied.
·about 3 days in 2 to 8 °C.
·Please avoid repeated freeze-thaw cycles.文献引用
1. Grinkova Y V, Denisov I G, Sligar S G. Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers[J]. Protein engineering, design & selection, 2010, 23(11): 843-848.
The soluble lipid bilayer nanodisc has proven to be a widely applicable method for membrane proteins as soluble in aqueous solutions, which can monodisperse and active membrane protein in a closely native bilayer environment. The key component of the nanodisc is the surrounding amphiphilic spiral protein band (membrane scaffold protein). Nanodisc systems have been used to reconstruction a variety of proteins including GPCRs, P450, bacterial rhodopsin, clotting factors, and tar receptors. Membrane scaffold protein 1E3 with larger inner diameter for twelve transmembrane membrane protein has been used as a scaffold protein for stabilizing lipid nanodiscs (ND). Membrane scaffold protein 1E3 (MSP1D1E3) is modified versions of apolipoprotein A1. It is a biparental synthetic protein that can self-assemble to form nanodiscs. The diameter of MSP1D1E3 is about 12 nm.
电泳
1μg (R: reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of MSP1D1E3 Protein his tag thrombin site in detergent is greater than 90% as determined by SEC-HPLC.
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