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分子别名
MSP1D1,MSP表达序列
Ser79-Gln267 with His tag and TEV site at N-Terminus表达宿主
E.coli分子量
22.87 kDa
纯度
＞95% by SDS-PAGE &＞95% by SEC-HPLC内毒素含量
<0.1EU/μg活性
The activity of MSP1D1 was performed by reconstructed with detergent. The peak volume of empty MSP1D1 in HPLC is about 5.2 ml, but the peak volume of MSP1D1 reconstructed detergent is about 4.6 ml, which shows that the MSP1D1 have reconstruction function.标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20 mM Tris, pH 8.0, 150 mM NaCl.
溶解方法
20 mM Tris, pH 8.0, 150 mM NaCl or PBS buffer, pH 7.4
储存条件
·6 months from date of receipt, -60 to -80 °C as supplied.
·about 3 days in 2 to 8 °C.
·Please avoid repeated freeze-thaw cycles.文献引用
1. Grinkova Y V, Denisov I G, Sligar S G. Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers[J]. Protein engineering, design & selection, 2010, 23(11): 843-848.
The soluble lipid bilayer nanodisc has proven to be a widely applicable method for membrane proteins as soluble in aqueous solutions, which can monodisperse and active membrane protein in a closely native bilayer environment. The key component of the nanodisc is the surrounding amphiphilic spiral protein band (membrane scaffold protein). Nanodisc systems have been used to reconstruction a variety of proteins including GPCRs, P450, bacterial rhodopsin, clotting factors, and tar receptors. Membrane scaffold protein 1D1 has been used as a scaffold protein for stabilizing lipid nanodiscs (ND). Membrane scaffold protein 1D1 (MSP1D1) is modified versions of apolipoprotein A1. It is a biparental synthetic protein that can self-assemble to form nanodiscs. The diameter of Nanodiscs is about 9.7 nm.
生物活性
The activity of MSP1D1 was performed by reconstructed with detergent. The peak volume of empty MSP1D1 in HPLC is about 5.2 ml, but the peak volume of MSP1D1 reconstructed detergent is about 4.6 ml, which shows that the MSP1D1 have reconstruction function.
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of MSP Protein his tag thrombin site in detergent is greater than 95% as determined by SEC-HPLC.
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