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物种
Rat分子别名
SGRF, IL-23p19, CLMF p40, IL-12 subunit p40, NKSF2, IL-23, Interleukin-23Accession
p19: Q91Z84, p40: Q9R278表达序列
p19: Val22-Ala196, with His Tag at N-terminus
p40: Met23-Ser335表达宿主
HEK293分子量
20-22, 40-45 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 =0.051 ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, 5% trehalose, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.·3 months, -20 to -80℃ under sterile conditions after reconstitution.·1 week, 2 to 8℃ under sterile conditions after reconstitution.·Please avoid repeated freeze-thaw cycles.
文献引用
Immunity 2000, 13(5):715-725.
J Biol Chem. 2020 Jul 24;295(30)
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
生物活性
Measured by its ability to induce IL-17 secretion by mouse splenocytes. The EC50 for this effect is 0.051 ng/mL.
电泳
2μg (R: reducing condition, N: non-reducing condition).
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