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物种
Human分子别名
Growth/differentiation factor 8, GDF-8, MyostatinAccession
O14793表达序列
Asp267-Ser375
表达宿主
CHO分子量
12-13 kDa (Reducing)
纯度
>95% by SDS-PAGE & HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 40-53 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
4 mM HCl
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in Reconstitution Buffer after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Nature. 1997;387(6628):83-90.
Nat Genet. 1997;17(1):71-74.
Growth differentiation factor-8 (GDF-8), commonly known as myostatin, is a ~25 kDa secreted glycoprotein belonging to the transforming growth factor-β (TGF-β) superfamily. GDF-8 is synthesized as a 376-amino acid prepropeptide, proteolytically processed to generate a latent prodomain-mature domain complex; activation requires further cleavage by tolloid-like proteases to release the biologically active mature dimer. The mature GDF-8 signals primarily through the activin type II receptor (ActRIIB), recruiting ALK4/ALK5 to activate Smad2/3 transcription factors, thereby suppressing myoblast proliferation, satellite cell activation, and muscle protein synthesis.
In 2004, a landmark case report described a human child with a homozygous myostatin mutation exhibiting gross muscle hypertrophy from infancy, providing direct proof-of-concept for therapeutic targeting in humans. Beyond muscle, GDF-8 influences adipogenesis (promoting fat accumulation), bone remodeling, cardiac hypertrophy, and tendon homeostasis.
生物活性
Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected IC50 for this effect is 40-53 ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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