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EGF Protein, Human

Pro-epidermal growth factor,EGF,Epidermal growth factor,Urogastrone

价格 ￥240.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040575
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone

  • Accession

    P01133

  • 表达序列

    Asn971-Arg1023

  • 表达宿主

    E.coli

  • 分子量

    10 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    IC50 = 2.3 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.
    Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-6

背景介绍

  • Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Epidermal growth factor (EGF) binds to epidermal growth factor receptor and stimulates an intracellular signal transduction cascade, leading to activation of genes that regulate cell proliferation, angiogenesis, motility, and metastasis. Epidermal growth factor (EGF) mRNA and protein are expressed in a number of adult tissues, especially in epithelial cells in the gastrointestinal tract. Predominant sites of synthesis of this peptide are the submandibular glands, the Brunner glands in the small intestine and the kidney.

  • 生物活性

    • Measured in a cell inhibition assay using A431 cell. The expected IC50 is 2.3 ng/ml.

    • EGF Protein, Human (Cat#UA040575) maintains colorectal cancer organoid growth well during multiple passages and long-term culture processes.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

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