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物种
Mouse分子别名
IFN-γ, Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gammaAccession
P01580表达序列
His23-Cys155
表达宿主
E.coli分子量
13 kDa (Reducing)
纯度
>95% by SDS-PAGE & HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 0.034-0.12 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20 mM Tris-HCl pH8.0, 300 mM NaCl, 5 % trehalose, 0.05 % Tween-20.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.·3 months, -20 to -80℃ under sterile conditions after reconstitution.·1 week, 2 to 8℃ under sterile conditions after reconstitution.·Please avoid repeated freeze-thaw cycles.
文献引用
Nat Methods. 2008 Dec;5(12):1011-7.
Adv Immunol. 2007:96: 41-101.
Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.
生物活性
Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected EC50 is 0.034-0.12 ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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