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产品中心 蛋白 细胞因子

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IL-27 Protein, Cynomolgus

Interleukin 27,IL27,IL-27

价格 ￥900.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040571
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Cynomolgus

  • 分子别名

    Interleukin 27, IL27, IL-27

  • 表达序列

    Q14213.2 EBI-3 Arg21-Lys229
    Q8NEV9.2 p28 Phe29-Pro243
    Linked by linker and followed by C-terminal His

  • 表达宿主

    HEK293

  • 分子量

    58-62 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 16 ng/ml

  • 标记

    Unconjugated

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, 5% trehalose, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Immunity, 16(6), 779-790.
    Journal of Experimental Medicine, 221(12).

背景介绍

  • Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family. It is composed of two subunits, Epstein-Barr virus-induced gene 3 (EBI3) and p28, and signals through a receptor complex formed by WSX-1 (also known as TCCR or IL-27Rα) and gp130.

    IL-27 is produced primarily by antigen-presenting cells (APCs), such as monocytes, dendritic cells, and macrophages, upon activation of pattern recognition receptors. The cynomolgus monkey (Macaca fascicularis) IL-27 protein shares a high degree of amino acid identity with its human ortholog (e.g., 92% for the p28 subunit), enabling the translation of findings from preclinical models.

    The biological effects of IL-27 are pleiotropic and context-dependent, integrating both pro-inflammatory and anti-inflammatory functions. It plays a key role in the early induction of Th1 differentiation and also exerts immunosuppressive effects. The specific outcome of IL-27 signaling—whether it promotes or resolves inflammation—is determined by the cellular context, disease state, and infection phase.

  • 生物活性

    • Measured in antiviral assays using HepG2 cells infected with vesicular stomatitis virus. The EC50 for this effect is 16 ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

  • ELISA

    • Immobilized IL-27 Protein, Cynomolgus (Cat. No. UA040571) at 2.0μg/mL (100μL/well) can bind IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, Human (Cat. No. UA011319) with EC50 of 25.5-37.6 ng/mL.

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