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产品中心 蛋白 细胞因子

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EGF Protein, Chicken

epidermal growth factor,EGF

价格 ￥260.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040568
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Chicken

  • 分子别名

    epidermal growth factor, EGF

  • Accession

    A0A8V0XLN7

  • 表达序列

    Gly1026-Arg1082

  • 表达宿主

    E.coli

  • 分子量

    13-15 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    IC50 < 10 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Proc Natl Acad Sci USA. 1975;72(4):1317-1321.
    Proc Natl Acad Sci USA. 2007;104(6):1771-1776.

背景介绍

  • Epidermal growth factor (EGF) is a 53-amino acid single-chain polypeptide (~6.2 kDa) with three intramolecular disulfide bonds that belongs to the EGF family of growth factors. It was first isolated by Stanley Cohen from mouse submaxillary glands in 1962, who observed that crude extracts caused precocious eyelid opening and tooth eruption in newborn mice; this work earned Cohen and Rita Levi-Montalcini the 1986 Nobel Prize in Physiology or Medicine. EGF exerts its biological effects by binding to the EGF receptor (EGFR/ErbB1/HER1), a receptor tyrosine kinase that triggers downstream signaling cascades (MAPK, PI3K/Akt) governing cell proliferation, differentiation, migration, and survival. Chicken EGF (gEGF) shares conserved structural features with mammalian homologs and has been successfully expressed in recombinant systems.

  • 生物活性

    • Measured by its ability to inhibit proliferation of A431. The IC50 for this effect is less than 10 ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

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