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KGF/FGF-7 Protein, Human

Fibroblast growth factor 7,FGF-7,Heparin-binding growth factor 7 (HBGF-7),Keratinocyte growth factor

价格 ￥1,920.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040561
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7 (HBGF-7), Keratinocyte growth factor

  • Accession

    P21781

  • 表达序列

    Cys32-Thr194

  • 表达宿主

    CHO

  • 分子量

    24-32 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Am J Transplant. 2021 Sep;21(9):2950-2963.
    Development. 1999 Feb;126(3):547-54.

背景介绍

  • Human fibroblast growth factor 7 (FGF-7) is also known as keratinocyte growth factor (KGF). It is a secreted, heparin-binding glycoprotein of 194 amino acids that belongs to the FGF family. FGF-7 is produced exclusively by mesenchymal cells and acts in a paracrine manner on epithelial cells expressing the splice variant FGFR2-IIIb. Upon ligand binding, the receptor dimerizes and initiates downstream signaling through the MAPK/ERK, PI3K/AKT and PLCγ pathways, leading to proliferation, migration, differentiation and survival of epithelial cells. Expression of FGF-7 is strongly up-regulated after skin, lung, kidney or gastrointestinal injury by inflammatory cytokines such as IL-1 and TNF-α, making it a key mediator of wound healing and tissue repair.

  • 生物活性

    • Measured in a cell proliferation assay using Hacat cells.

    • KGF/FGF-7 Protein, Human (Cat#UA040561) maintains lung cancer organoid growth well during multiple passages and long-term culture processes.

    • KGF/FGF-7 Protein, Human (Cat#UA040561) maintains bud tip progenitor organoid growth well during multiple passages and long-term culture processes.

    • Determined by a cell proliferation assay using 4MBr-5 cells. The expected EC50 for this effect is 3.6-8.7ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

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