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Noggin Protein, Human

Noggin,SYM1,SYNS1,NOG

价格 ￥700.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040560
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Noggin, SYM1, SYNS1, NOG

  • Accession

    Q13253

  • 表达序列

    Gln28-Cys232

  • 表达宿主

    CHO

  • 分子量

    26-35 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 59 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, 5% Trehalose, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Cell. 1996 Aug 23;86(4):599-606.
    Nature. 2002 Dec 12;420(6916):636-42.

背景介绍

  • Human Noggin is a secreted homodimeric glycoprotein encoded by the NOG gene (17q22). Originally identified in Xenopus as the dorsalizing factor from Spemann’s organizer, it is a pivotal antagonist of bone morphogenetic proteins (BMPs). Noggin is essential for embryonic axis formation, neural induction, somite patterning, and joint morphogenesis; loss-of-function mutations in humans cause proximal symphalangism (SYM1) and multiple synostoses syndrome.

    Noggin binds BMP-2, BMP-4, and BMP-7 with high affinity, physically blocking their interaction with type I and type II serine/threonine kinase receptors. This prevents receptor oligomerization and downstream SMAD1/5/8 phosphorylation, thereby inhibiting BMP-driven transcriptional programs. Its cystine-knot structure confers thermal stability and potent antagonistic activity, making recombinant human Noggin a standard reagent in stem-cell and developmental biology.

  • 生物活性

    • Measured by its ability to inhibit the activation of Human BMP (Luc) HEK293T Reporter cell by Human BMP-2 Protein. The EC50 for this effect is 59 ng/ml.

    • Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected IC50 for this effect is 0.89-1.8 ng/ml of Noggin.

    • Noggin Protein, Human (Cat#UA040560) maintains lung cancer organoid growth well during multiple passages and long-term culture processes.

    • Noggin Protein, Human(Cat#UA040560) maintains colorectal cancer organoid growth well during multiple passages and long-term culture processes.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

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