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LR3-IGF-I Protein, Salmon

LR3 Insulin-like Growth Factor-I,MGF,IGFI,IGF1A,IGF,IBP1

价格 ￥560.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040556
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Salmon

  • 分子别名

    LR3 Insulin-like Growth Factor-I, MGF, IGFI, IGF1A, IGF, IBP1

  • Accession

    Q91476

  • 表达序列

    Gly19-Ala88 (Glu21Arg)

  • 表达宿主

    E.coli

  • 分子量

    10-12 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 0.71-1.8 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Endocrinology. 1999 Oct;140(10):4699-705.
    J Neurotrauma. 2018 Jul 1;35(13):1467-1480.

背景介绍

  • IGF-I is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-I is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-II, which is also a major fetal growth factor. Salmon Long R3 Insulin-like Growth Factor-I contains a 70 amino acid analog of Salmon IGF-I. Compared to the complete IGF-I sequence, an addition of the LR3-IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3) and a 13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of Long R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.

  • 生物活性

    • Determined by a cell proliferation assay using serum free human MCF-7 cells. The EC50 is 0.71-1.8 ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

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