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FGF-basic Protein, Chicken

Fibroblast growth factor 2,FGF-2,Basic fibroblast growth factor (bFGF),Heparin-binding growth factor 2 (HBGF-2)

价格 ￥1,200.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040550
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Chicken

  • 分子别名

    Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor (bFGF), Heparin-binding growth factor 2 (HBGF-2)

  • Accession

    P48800

  • 表达序列

    Pro13-Ser158

  • 表达宿主

    E.coli

  • 分子量

    14-15 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 0.030-0.061 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    iScience. 2022 Sep 3;25(10):105054.
    J Bone Miner Res. 1994 Mar;9(3):431-9.

背景介绍

  • Basic fibroblast growth factor (bFGF), also known as FGF-2 or FGF-β, is a member of the fibroblast growth factor family that regulates essential biological functions including cell proliferation, survival, migration, and differentiation. The FGF family comprises 18-22 members grouped into subfamilies based on sequence homology, with FGF-2 binding to and signaling through all four FGF receptors (FGFR1-4), transmembrane receptors with intracellular tyrosine kinase activity. Upon activation, FGFRs recruit SH2 or PTB domain-containing signaling proteins, leading to activation of downstream pathways including RAS/MAP kinase, PI3 kinase/Akt, and PLCγ.

    The industrial applications of chicken bFGF have expanded dramatically with the emergence of cellular agriculture and cultivated meat production. Recombinant chicken/duck FGF-2 protein (145 aa) is specifically developed for species-specific avian cellular agriculture protocols, serving as a critical medium component for cultivating chicken cells for food applications.

  • 生物活性

    • Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is 0.030-0.061 ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

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