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物种
Human分子别名
bFGF, FGF-2, Heparin-binding growth factor 2, Basic fibroblast growth factor, FGFB, HBGF-2, FGF2, Fibroblast Growth Factor 2 (Basic)Accession
P09038表达序列
Ala135-Ser288 with proprietary amino acid modifications
表达宿主
E.coli分子量
14-15 kDa (Reducing)
纯度
>95% by SDS-PAGE & HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 0.0091-0.019 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
1. J Biol Chem. 1996 Jun 21;271(25):15292-7.
2. Biosci Rep. 2017 Apr 10;37(2): BSR20170173.Print 2017 Apr 28.
3. Biochem Biophys Res Commun. 1986 Mar 13;135(2):541-8.
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
生物活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells, the EC50 for this effect is 0.0091-0.019ng/ml.
*Recombinant FGF-basic Heat Stable protein was incubated at 4°C or 37°C for 3 days in media. The protein retained similar activity indicating that the Heat Stable protein
has increased thermal stability.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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