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物种
Human分子别名
Fibronectin Curated, FN, Cold-insoluble globulin (CIG)Accession
P02751-15表达序列
Pro1361-Ser1637&Ala1812-Thr2107
表达宿主
E.coli分子量
58-68 kDa (Reducing)
纯度
>95% by SDS-PAGE & HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 1 μg/mL标记
Unconjugated标签
No Tag性状
Liquid缓冲体系
12.5 mM sodium citrate, 1.25% sucrose, pH 6.2
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
文献引用
Nature. 1990 Jun 14;345(6276):642-6.
Nat Rev Immunol. 2006 May;6(5):383-93.
Fibronectin-CH296 is a recombinant human fibronectin fragment developed in 1995. The protein comprises 574 amino acids with a molecular weight of 58–63 kDa, produced in E. coli expression systems. CH296 contains three critical functional domains derived from the parent fibronectin molecule:
1. Central Cell-Binding Domain (type III repeats 8-10): Contains the Arg-Gly-Asp (RGD) sequence that binds integrin VLA-5 (α5β1) on target cells.
2. Heparin-Binding Domain II (type III repeats 12-14): Mediates binding to viral particles via heparan sulfate moieties on retroviral/lentiviral envelopes.
3. CS-1 Site (alternatively-spliced IIICS region): Binds integrin VLA-4 (α4β1) expressed on hematopoietic cells, T cells, and monocytes. This domain organization enables co-localization of viral particles and target cells on the CH296-coated surface, dramatically enhancing transduction efficiency by increasing effective viral concentration at the cell membrane.
生物活性
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The EC50 for this effect is less than 1 μg/mL.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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