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IGFBP-6 Protein, Mouse

Insulin-like growth factor-binding protein 6,IBP-6,IGF-binding protein 6,IGFBP-6

价格 ￥700.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040535
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    Insulin-like growth factor-binding protein 6, IBP-6, IGF-binding protein 6, IGFBP-6

  • Accession

    P47880

  • 表达序列

    Ala26-Gly238 with His Tag at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    30-35 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 0.286-0.831 μg/ml

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, 1mM EDTA, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    J Clin Invest. 2008 Jul;118(7):2609-19.
    Growth Horm IGF Res. 2016 Oct-Dec:30-31:81-86.

背景介绍

  • Insulin-like Growth Factor Binding Protein-6 (IGFBP-6) is a 30-kDa secreted glycoprotein encoded by the Igfbp6 gene, distinguished by its extraordinary ~50–70-fold binding preference for IGF-II over IGF-I. This selectivity is mediated by cooperative interactions between the N-terminal subdomain and C-terminal domain of IGFBP-6 with adjacent binding sites on IGF-II. IGFBP-6 primarily functions as a selective inhibitor of IGF-II actions, blocking IGF-II-induced cell proliferation, differentiation, migration, and survival across diverse cell types including myoblasts, neuroblastoma cells, and rhabdomyosarcoma cells. Unlike other IGFBPs, it has minimal effect on IGF-I activity.

    IGFBP-6 expression is developmentally regulated in mouse skeletal muscle, with high levels during embryogenesis and sustained expression in adult muscle tissue. In aging mice, serum IGFBP-6 levels increase progressively. Expression is regulated by TGF-β (via SMAD2/3), hedgehog signaling, p53, and hypoxia-inducible factors.

  • 生物活性

    • Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. The EC50 is 0.286-0.831 μg/mL in the presence of 14 ng/mL human IGF-II.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

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