热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 蛋白 细胞因子

所有图片（1/3）

IGFBP-4 Protein, Human

Insulin-like growth factor-binding protein 4,IBP-4,IGF-binding protein 4,IGFBP-4

价格 ￥700.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040534
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4

  • Accession

    P22692

  • 表达序列

    Asp22-Glu258 with His Tag at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    35-38 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 =31-48 ng/ml

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, 1mM EDTA, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    J Clin Invest. 2008 Jul;118(7):2609-19.
    Circ Res. 2000 Feb 4;86(2):125-30.

背景介绍

  • Insulin-like Growth Factor Binding Protein-4 (IGFBP-4) is the smallest member of the IGFBP family, with a molecular weight of approximately 24 kDa, encoded by the IGFBP4 gene located on human chromosome 17q12-21.1. The protein consists of 237 amino acids and contains the conserved N-terminal, C-terminal, and variable L-domains characteristic of the IGFBP family, including two additional interconnected cysteine residues in the L-domain. IGFBP-4 binds both IGF-I and IGF-II with high affinity, exhibiting 2–10 fold higher affinity for IGF-II than for IGF-I. This binding prolongs IGF half-life and inhibits ligand-receptor interactions, thereby attenuating IGF-mediated proliferation and differentiation in most physiological contexts.

  • 生物活性

    • Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The EC50 for this effect is typically 31-48ng/mL in the presence of 14 ng/mL human IGFII.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

评论(0)