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R-spondin-1 Protein, Mouse STAR

R-spondin-1,Cysteine-rich and single thrombospondin domain-containing protein 3 (Cristin-3,mCristin-3),Roof plate-specific spondin-1,RSPO1

价格 ￥680.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040525
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    R-spondin-1, Cysteine-rich and single thrombospondin domain-containing protein 3 (Cristin-3; mCristin-3), Roof plate-specific spondin-1,RSPO1

  • Accession

    Q9Z132

  • 表达序列

    Ser21-Gln265

  • 表达宿主

    HEK293

  • 分子量

    38-40 kDa (Reducing)

  • 纯度

    >95% by Bis-Tris PAGE & HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 18-28 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Nature. 2011 Jan 20;469(7330):415-8.
    Nat Biotechnol. 2017 Jun;35(6):577-582.

背景介绍

  • The R-spondin (RSPO) family comprises four secreted glycoproteins (RSPO1-4) that function as critical modulators of canonical Wnt/β-catenin signaling. These proteins are characterized by two N-terminal furin-like cysteine-rich domains and a C-terminal thrombospondin type 1 repeat (TSR-1) motif. Mouse models have been instrumental in elucidating RSPO functions, revealing their essential roles in developmental morphogenesis, adult stem cell maintenance, and disease pathogenesis through interactions with LGR4/5/6 receptors and regulation of Wnt receptor availability.
    Industrially, recombinant mouse RSPO proteins have become indispensable components in organoid culture systems for drug screening and personalized medicine. Pharmaceutical companies utilize RSPO-based platforms to generate patient-derived intestinal, gastric, hepatic, mammary, and prostate organoids for high-throughput drug testing. Therapeutically, RSPO1 shows promise for treating inflammatory bowel disease, chemotherapy-induced mucositis, and short bowel syndrome by promoting intestinal regeneration. Conversely, anti-RSPO antibodies are under development as cancer therapeutics for RSPO-fusion-positive tumors, representing a targeted approach in precision oncology.

  • 生物活性

    • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical EC50 is 18-28ng/ml in the presence of 5ng/ml Recombinant Human Wnt Surrogate (UA040250).

    • Mouse bile duct organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), FGF-10(UA040076), HGF(UA040194) and Wnt Surrogate(UA040250).

    • Mouse lung organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), FGF-10(UA040076), HGF(UA040194), Wnt Surrogate(UA040250) and KGF/FGF-7(UA040110)

    • Mouse Gastric organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), Wnt Surrogate(UA040250), KGF/FGF-7(UA040110) and FGF-basic(UA040339)

    • Mouse small intestinal organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), Wnt Surrogate(UA040250).

    • Mouse Colon organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), Wnt Surrogate(UA040250).

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

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