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物种
Mouse分子别名
Glial cell line-derived neurotrophic factor, mGDNF, Astrocyte-derived trophic factor (ATF)Accession
P48540表达序列
Ser78-Ile211
表达宿主
E.coli分子量
12-14 kDa (Reducing)纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
EC50 = 2.9-9.8 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Science. 1993;260(5111):1130-1132.
Nat Rev Neurosci. 2002;3(5):383-394.
Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor belonging to the TGF-β superfamily, first isolated in 1993 as a survival factor for midbrain dopaminergic neurons. In mice, GDNF is encoded by the Gdnf gene on chromosome 15 and expressed as a 211-amino acid preproprotein that undergoes proteolytic processing to form the active disulfide-linked homodimer. The mature mouse GDNF protein (Ser78-Ile211) signals through a multicomponent receptor system comprising the RET receptor tyrosine kinase and one of four GFRα co-receptors (GFRα1-4). This unique signaling architecture mediates GDNF's pleiotropic functions in neuronal survival, differentiation, dopamine uptake enhancement, and axonal guidance. Beyond the central nervous system, mouse GDNF is essential for kidney organogenesis, enteric nervous system development, and spermatogonial stem cell self-renewal, demonstrating evolutionary conserved roles across organ systems. Homozygous Gdnf knockout mice exhibit neonatal lethality with renal agenesis and intestinal atresia, while conditional knockouts reveal critical requirements for adult dopaminergic neuron maintenance and motor control.
生物活性
Measured in a cell proliferation assay using SHSY5Y human neuroblastoma cells. The EC50 for this effect is less than 2.9-9.8ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized GDNF Protein, Mouse (Cat. No. UA040523) at 1.0μg/mL (100μL/well) can bind GFRA1 Fc Chimera Protein, Human (Cat. No. UA011102) with EC50 of 0.81-1.67ng/mL.
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