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IGFBP-1 Protein, Human

Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Placental protein 12 (PP12)

价格 ￥1,850.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040519
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12 (PP12)

  • Accession

    P08833

  • 表达序列

    Ala26­Asn259, with C-terminal His tag

  • 表达宿主

    HEK293

  • 分子量

    35-38 kDa (Reducing)

  • 纯度

    >95% by Bis-Tris PAGE and HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 11.93-16.50ng/mL

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Trends Endocrinol Metab. 2009 May;20(4):153-62.
    Cell Metab. 2015 Nov 3;22(5):811-24.

背景介绍

  • IGFBP-1 is a secreted protein encoded by the IGFBP1 gene located on chromosome 7 in humans. It is a member of the insulin-like growth factor binding protein family, which consists of six high-affinity binding proteins (IGFBP-1 to -6).
    Human IGFBP-1 has evolved from being viewed as a passive IGF carrier to being recognized as a dynamic, multifunctional protein with significant IGF-independent biological activities. Its primary research utility lies in its role as a sensitive biomarker for hepatic insulin sensitivity and pregnancy-related conditions. Industrially, it is harnessed for diagnostic immunoassays and explored as a novel therapeutic target. Future research is likely to further dissect its tissue-specific roles and translational potential in metabolic, bone, and neoplastic diseases.

  • 生物活性

    • Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. The ED50 for this effect is 44-139 ng/mL in the presence of 6 ng/mL recombinant human IGF-I

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

  • ELISA

    • Immobilized IGFBP-1 Protein, Human (Cat. No. UA040519) at 2.0μg/mL (100μL/well) can bind IGF-I Protein, Human (Cat. No. UA040520) with EC50 of 11.93-16.50ng/mL.

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