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IL-6 Protein, Rat

Interleukin-6,IL-6

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040507
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Rat

  • 分子别名

    Interleukin-6, IL-6

  • Accession

    P20607

  • 表达序列

    Phe25-Thr211

  • 表达宿主

    E.coli

  • 分子量

    20-22 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE and HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 51-78pg/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM NaAc, 300mM NaCl, pH5.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Semin Immunol. 2014 Feb;26(1):2-12.
    Nat Rev Rheumatol. 2014 Dec;10(12):720-7.

背景介绍

  • Rat Interleukin-6 (IL-6) is a pleiotropic cytokine encoded by the Il6 gene on chromosome 4 (NC_086022.1, 5889999..5894575, complement), consisting of 5 exons that produce a protein-coding transcript . As a key member of the IL-6 family, it orchestrates acute-phase responses, immune regulation, and metabolic homeostasis in rodents. Signals through two distinct pathways in rats: Classic signaling: IL-6 binds membrane-bound IL-6Rα, recruiting gp130 to activate JAK1/STAT3 cascade; Trans-signaling: Soluble IL-6Rα (sIL-6R) complexes with IL-6 to activate gp130 on cells lacking membrane IL-6Rα, amplifying inflammatory responses in neurons and vascular tissue.

  • 生物活性

    • Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The EC50 for this effect is 51-78pg/mL.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

  • ELISA

    • Immobilized IL-6 Protein, Rat (Cat. No. UA040507) at 5.0μg/mL (100μL/well) can bind IL-6R alpha/CD126 Fc Chimera Protein, Mouse (Cat. No. UA011252) with EC50 of 38.5-192.3 ng/mL.

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