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VEGF 120 Protein, Mouse

Vascular endothelial growth factor A,long form,L-VEGF,Vascular permeability factor (VPF),VEGFA

价格 ￥1,060.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040501
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    Vascular endothelial growth factor A, long form, L-VEGF, Vascular permeability factor (VPF), VEGFA

  • Accession

    Q00731

  • 表达序列

    Ala27-Arg146

  • 表达宿主

    E.coli

  • 分子量

    15-16 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE; >90% by HPLC;

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 2.92-5.46 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Nature. 1996 Apr 4;380(6573):435-9.
    Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):761-6.

背景介绍

  • VEGF 120 is the smallest functional isoform of Vascular Endothelial Growth Factor A (VEGF-A) in mice, generated through alternative splicing of the Vegfa gene. Unlike the larger isoforms (VEGF 164 and VEGF 188), VEGF 120 lacks exons 6 and 7, which encode heparin-binding domains and extracellular matrix (ECM) interaction sites. This structural deficiency makes VEGF 120 the most soluble and freely diffusible isoform, unable to bind heparan sulfate proteoglycans (HSPGs) or ECM components.
    The diffusible nature of VEGF 120 results in a broad, diffuse immunostaining pattern in developing tissues, contrasting with the steep, localized gradients formed by ECM-binding isoforms. In the developing mouse forebrain, VEGF 120 contributes to the dual regulation of neurogenesis and angiogenesis. Expressed by radial glia and neurons, its freely diffusible properties allow it to influence neural stem cell (NSC) proliferation and differentiation across larger tissue volumes compared to matrix-bound isoforms.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >90% as determined by RP-HPLC.

  • ELISA

    • Immobilized VEGF-120 Protein, Mouse (Cat. No. UA040501) at 2.0μg/mL (100μL/well) can bind VEGF-R2/KDR Fc Protein, Human (Cat. No. UA010144) with EC50 of 2.92-5.46 ng/mL.

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