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TGF-β2 Protein, Human

Transforming growth factor beta-2 proprotein,Cetermin,Glioblastoma-derived T-cell suppressor factor,G-TSF

价格 ￥2,650.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040490
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Transforming growth factor beta-2 proprotein, Cetermin, Glioblastoma-derived T-cell suppressor factor, G-TSF

  • Accession

    P61812

  • 表达序列

    Ala303-Ser414

  • 表达宿主

    HEK293

  • 分子量

    12 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE and HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 0.2 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    4mM HCl

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in reconstitution buffer after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Signal Transduct Target Ther. 2024 Mar 22;9(1):61.
    Cytokine Growth Factor Rev. 2006 Feb-Apr;17(1-2):129-39.

背景介绍

  • TGF-β2 (Transforming Growth Factor-beta 2) is a secreted, 25 kDa disulfide-linked homodimeric cytokine and one of three mammalian TGF-β isoforms. It is synthesized as a 414-aa pre-pro-peptide; after removal of the signal peptide and cleavage of the latency-associated peptide (LAP), the mature 112-aa chain forms the biologically active ligand. TGF-β2 is abundant in bone, cartilage, cornea, kidney, platelets and immune cells, and its expression is rapidly up-regulated after injury or in fibrotic disease. Compared with TGF-β1, TGF-β2 exhibits ~71 % sequence identity but shows distinct promoter regulation and a stronger association with epithelial and neuronal tissues.
    TGF-β2 signals through the canonical type II–I receptor serine/threonine kinases TβRII and ALK5, phosphorylating Smad2/3. Phospho-Smad complexes with Smad4 translocate to the nucleus to regulate >500 genes. Non-Smad pathways—PI3K/Akt, mTORC2, MAPK, JAK/STAT3, Rho-GTPases—mediate additional effects on cell survival, migration and EMT.

  • 生物活性

    • Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The EC50 for this effect is less than 0.2ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by RP-HPLC.

  • SPR

    • Protein A Chip captured TGFBR2 Fc Chimera Protein, Human (Cat. No. UA010650), can bind TGF-β2 Protein, Human (Cat. No. UA040490) with an affinity constant of 5.73nM as determined in SPR assay.

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