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物种
Mouse分子别名
Vascular endothelial growth factor A, long form; L-VEGF 1; Vascular permeability factor (VPF),VEGFAAccession
Q00731-2表达序列
Ala27-Arg190
表达宿主
E.coli分子量
20-22 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 3.36-4.41 ng/mL标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Nat Med. 2003 Jun;9(6):669-76.
Nature. 1996 Apr 4;380(6573):435-9.
VEGF-A164 (often written VEGF164) is the predominant splice variant of vascular endothelial growth factor A in the mouse. It is a heparin-binding, disulfide-linked homodimer of 19–22 kDa that is generated by alternative splicing of the Vegfa gene and corresponds to human VEGF-A165. VEGF-A164 is expressed in most embryonic and adult tissues, with especially high levels in the placenta, brain (choroid plexus), kidney and heart. It signals primarily through the receptor tyrosine kinases VEGFR-1 (Flt-1) and VEGFR-2 (Flk-1/KDR) to promote endothelial-cell proliferation, migration, vascular permeability and survival. Global or endothelial-specific Vegfa knockout is embryonic-lethal, underscoring its indispensable role in developmental angiogenesis and organogenesis.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
ELISA
Immobilized VEGF-A164 Protein, Mouse (Cat. No. UA040486) at 2.0μg/mL (100μL/well) can bind VEGF R2/Flk-1/KDR Fc Chimera Protein, Mouse (Cat. No. UA010876) with EC50 of 3.36-4.41 ng/mL.
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