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物种
Mouse分子别名
Klotho; EC:3.2.1.31Accession
O35082表达序列
Ala35-Lys982, with C-terminal His Tag
表达宿主
HEK293分子量
65, 75, 120-130 kDa (Reducing)
纯度
>85% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 53.47-67.59 ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, 5% trehalose, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Nature. 1997 Nov 6;390(6655):45-51.
Science. 2005 Sep 16;309(5742):1829-33.
Mouse Klotho (mKL) is a 1014-aa type-I trans-membrane glycoprotein of the glycosidase-1 super-family that is expressed mainly in distal convoluted tubule cells of the kidney, choroid plexus of the brain, and adrenal zona glomerulosa. A soluble 130-kDa form is generated by ADAM10/17 proteolytic shedding and circulates in plasma and CSF. Klotho functions as an obligate co-receptor for FGF23, thereby regulating phosphate and vitamin-D homeostasis; It also exhibits intrinsic β-glucuronidase activity that activates TRPV5/6 channels for renal Ca²⁺ reabsorption. Homozygous Klotho-deficient (kl/kl) mice on a mixed C57BL/6J, FVB, and C3H/J background develop a premature-aging phenotype—growth retardation, osteoporosis, arteriosclerosis, skin atrophy, emphysema, infertility, and death by 8–9 weeks. Conversely, transgenic over-expression extends lifespan and improves cognition, synaptic plasticity, and stress resilience.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>85% as determined by RP-HPLC.
ELISA
Immobilized rHuFGF-23 at 5.0μg/mL (100μL/well) can bind Klotho Protein, Mouse (Cat. No. UA040485) with EC50 of 53.47-67.59 ng/mL.
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