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物种
Mouse分子别名
Interleukin-4; IL-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1 (BSF-1); IGG1 induction factor; Lymphocyte stimulatory factor 1;Accession
P07750表达序列
His23-Ser140
表达宿主
HEK293分子量
17-20 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Med Sci Monit. 2016 Jan 23:22:244-50.
Blood. 2009 Nov 12;114(20):4517-26.
Mouse Interleukin-4(IL-4) is a monomeric, Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α-helix structure. IL-4 exerts its effects through two receptor complexes, Participates in at least several B-cell activation processes as well as of other cell types. IL-4 is primarily expressed by Th2-biased CD4+T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by CD4+T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
流式分析
Flow cytometry detection results of M2 polarization in mouse RAW264.7 cells
生物活性
Measured in a cell proliferation assay using mouse HT-2 cells, the EC50 for this effect is less than 2ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
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