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物种
Mouse, Rat分子别名
Transforming growth factor beta-1; TGF-β1Accession
P04202表达序列
Ala279-Ser390
表达宿主
HEK293分子量
12-13 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 0.1 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
4mM HCl
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in 4mM HCl after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Annu Rev Immunol. 2006:24:99-146.
ISRN Mol Biol. 2012 Dec 24:2012:381428.
Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factors and plays a prominent role in a variety of cellular processes, including cell-cycle progression, cell differentiation, reproductive function, development, motility, adhesion, neuronal growth, bone morphogenesis, wound healing, and immune surveillance. TGF-β1, TGF-β2 and TGF-β3 signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.
生物活性
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The EC50 for this effect is less than 0.1 ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
ELISA
Immobilized TGF-β1 Protein, Mouse/Rat (Cat. No. UA040459) at 0.2μg/mL (100μL/well) can bind TGFBR2 Fc Chimera Protein, Mouse (Cat. No. UA010646) with EC50 of 2.95-5.07ng/mL.
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