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物种
Human分子别名
IL-27 p28 subunit,IL-27 subunit alpha,IL27,IL-27,IL27A,IL-27-A,IL27-A,IL27p28,IL30,interleukin 27,interleukin 30,interleukin-27 subunit alpha,MGC71873,p28IL-27A表达序列
Arg21-Lys229 (Q14213)
Phe29-Pro243 (Q8NEV9)表达宿主
HEK293分子量
27-33 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 50ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH 7.4, 5%Trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Annu Rev Immunol. 2015:33:417-43.
J Neuroinflammation. 2022 Sep 1;19(1):212.
Interleukin-27 (IL-27) is a heterodimeric cytokine that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of two subunits: EBI3 (EBV-induced gene 3), which is related to the p40 subunit of IL-12 and IL-23, and p28, which is related to the p35 chain of IL-12. IL-27 is primarily produced by monocytes, endothelial cells, and dendritic cells. It signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. IL-27 has both anti-inflammatory and pro-inflammatory properties. As an anti-inflammatory cytokine, it induces a general negative feedback program that limits T and NK-T cell activity. During the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity and possible Th1 development.
生物活性
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected EC50 is less than 50 ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized IL-27 Protein, Human (Cat. No. UA040457) at 2.0μg/mL (100μL/well) can bind IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, Human (Cat. No. UA011319) with EC50 of 10.03-15.25 ng/mL.
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