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物种
Mouse分子别名
R-spondin-2,Cysteine-rich and single thrombospondin domain-containing protein 2 (Cristin-2,mCristin-2),Roof plate-specific spondin-2Accession
Q8BFU0表达序列
Ala32-Gly205, with C-terminal hIgG1 Fc Tag
表达宿主
HEK293分子量
46-55 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 10 ng/ml标记
Unconjugated性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Development. 2008 Mar;135(6):1049-58.
Mol Biol Cell. 2008 Jun;19(6):2588-96.
R-Spondin2 (RSPO2) is a member of the R-Spondin family and is a secreted protein that plays a crucial role in regulating Wnt/β-catenin signaling. In mice, R-Spondin2 is essential for various developmental processes. For example, it is required for the maintenance of the apical ectodermal ridge in the hindlimb, which is critical for proper limb development. Additionally, R-Spondin2 is involved in the development of the larynx, trachea, lung, and other organs. It also plays a role in bone formation and mineralization by enhancing Wnt/β-catenin signaling.
R-Spondin2's ability to enhance Wnt signaling makes it a potential candidate for promoting tissue regeneration and repair. It can be used to stimulate osteoblast differentiation and bone formation, which is beneficial for treating bone-related disorders. Also, R-Spondin2 is involved in the regulation of stem cell proliferation and differentiation. Its role in Wnt signaling can be exploited to study cancer stem cells and develop targeted therapies for cancers that involve dysregulated Wnt pathways
生物活性
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical EC50 is less than 10ng/ml in the presence of 5ng/ml Recombinant Human Wnt Surrogate.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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