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物种
Mouse分子别名
Thrombopoietin,C-mpl ligand (ML),Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor (MGDF),Myeloproliferative leukemia virus oncogene ligand,Accession
P40226表达序列
Ser22Thr356, with C-terminal His Tag表达宿主
HEK293分子量
72-95 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 < 1.0ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Science. 2018 Apr 6;360(6384):106-110.
Biochem Pharmacol. 2024 Feb: 220:116008.
Thrombopoietin (TPO) is the primary cytokine responsible for regulating platelet production by stimulating the proliferation and differentiation of megakaryocytes, the bone marrow cells that produce platelets. TPO binds to the thrombopoietin receptor (TPO-R or c-Mpl) on hematopoietic stem cells (HSCs) and megakaryocyte progenitors, activating signaling pathways such as JAK2/STAT5, PI3K/AKT, and RAF-1/MAPK, which promote platelet production and maintain hematopoietic stem cell populations.
TPO and its receptor agonists (TPO-RAs) are widely used to treat immune thrombocytopenia (ITP), chemotherapy-induced thrombocytopenia, and thrombocytopenia associated with chronic liver disease or myelodysplastic syndromes (MDS)37. Recombinant human TPO (rhTPO) and synthetic TPO-RAs (e.g., romiplostim, eltrombopag, avatrombopag) have shown efficacy in increasing platelet counts in clinical settings.
生物活性
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is less than 1ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized TPO (335aa) Protein, Mouse (Cat. No. UA040417) at 0.5μg/mL (100μL/well) can bind Biotinylated Thrombopoietin R/TPO R His Tag Protein, Mouse with EC50 of 0.44-1.02ug/mL.
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