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物种
Mouse分子别名
Thrombopoietin,C-mpl ligand (ML),Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor (MGDF),Myeloproliferative leukemia virus oncogene ligand,Accession
P40226表达序列
Ser22-Phe195, with C-terminal His Tag
表达宿主
HEK293分子量
26-29 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 0.2ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Autoimmunity. 2012 Sep;45(6):476-84.
J Immunol. 2001 Jan 15;166(2):1327-33.
Thrombopoietin (TPO) is a glycoprotein hormone that plays a crucial role in the regulation of platelet production by stimulating the proliferation and differentiation of megakaryocytes. The 174 amino acid (aa) form of TPO is a key isoform that has been extensively studied for its biological activities. In mice, TPO is essential for maintaining normal platelet counts and has been used as a model to understand its functions in hematopoiesis and thrombopoiesis.
TPO (174aa) is a target for the development of therapeutic agents aimed at treating thrombocytopenia and other platelet disorders. Recombinant TPO analogs and TPO receptor agonists are being developed and tested for their efficacy in increasing platelet counts. Also, TPO levels can be used as a biomarker for certain hematological conditions. Assays and kits for measuring TPO in biological samples are being developed for diagnostic purposes.
生物活性
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is less than 0.2ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
ELISA
Immobilized TPO Protein(174aa), Mouse His Tag (Cat. No. UA040416) at 0.5μg/mL (100μL/well) can bind Biotinylated Thrombopoietin R/TPO R His Tag Protein, Mouse with EC50 of 0.69-1.00ug/mL.
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