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物种
Human分子别名
Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2;Accession
P18065表达序列
Ala36-Gln325, with C-terminal His Tag
表达宿主
CHO分子量
34-36 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 96-116 ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3475-80.
Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5563-8.
Insulin-like growth factor-binding protein-2 (IGFBP-2) is a 36-kDa cysteine-rich secreted glycoprotein that preferentially binds IGF-II and is highly expressed during embryogenesis, especially in the central nervous system and reproductive tissues. Beyond simply sequestering IGFs, IGFBP-2 possesses intrinsic, IGF-independent activities: it contains an RGD integrin-binding motif, is internalized via a nuclear-localization signal, and can activate PI3K/Akt, NF-κB and β-catenin pathways. Consequently, IGFBP-2 functions as a developmental regulator and, when aberrantly re-activated, as an oncogenic hub that promotes epithelial-to-mesenchymal transition, angiogenesis, stemness and chemo-resistance in multiple solid tumours.
生物活性
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. The ED50 for this effect is 11-26 ng/mL in the presence of 14 ng/mL rhIGF-II.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
ELISA
Immobilized IGF-I Protein, Human at 5.0μg/mL (100μL/well) can bind IGFBP-2 Protein, Human His Tag (Cat. No. UA040414) with EC50 of 0.12-0.16ug/ml.
Immobilized IGF-II Protein, Human (Cat. No. UA040162) at 5.0μg/mL (100μL/well) can bind IGFBP-2 Protein, Human His Tag (Cat. No. UA040414) with EC50 of 0.095-0.116ug/ml.
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