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物种
Mouse分子别名
Tumor necrosis factor ligand superfamily member 13B,B-cell-activating factor,BAFF,CD257Accession
Q9WU72表达序列
Ala127-Leu309
表达宿主
HEK293分子量
20-22 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 = 0.013-0.035μg/ml.标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 500mM NaCl, pH7.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
MedComm (2020). 2023 May 17;4(3):e274.
Int J Mol Sci. 2021 Jan 16;22(2):861.
BAFF, also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein belonging to the TNF ligand superfamily. It plays a crucial role in the survival, proliferation, and differentiation of B cells. Mouse BAFF shares 72% amino acid sequence identity with human BAFF within its extracellular domain. This cytokine is produced by various hematopoietic cells, including monocytes, macrophages, neutrophils, dendritic cells, T cells, and adipocytes. BAFF binds to three receptors primarily expressed by B cells: BAFF-R, TACI, and BCMA. Elevated BAFF levels are associated with autoimmune diseases and B cell malignancies.
BAFF has gained attention in the field of organoid culture due to its critical role in B cell biology and its potential to influence the immune microenvironment within organoids. For example, in organoids derived from lymphoid tissues, BAFF can help maintain B cell viability and support the formation of germinal centers.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Mouse BAFF is immobilized 2µg/mL (100 µl/well), Recombinant Mouse BAFFR (UA010419) binds with an EC50 of 0.013-0.035μg/ml.
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