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物种
Mouse分子别名
NogginAccession
P97466表达序列
Gln28-Cys232
表达宿主
CHO分子量
29-32 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 64-77 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Nature. 2000 Feb 10;403(6770):658-61.
Science. 1998 May 29;280(5368):1455-7.
Mouse Noggin is a 64 kDa secreted homodimeric glycoprotein that functions as a potent antagonist of Bone Morphogenetic Protein (BMP) signaling. Encoded by the Nog gene, Noggin directly binds BMP ligands (BMP-2, -4, -5, -7) and Growth Differentiation Factors (GDF-5, -6) with high affinity, preventing their interaction with cognate receptors and subsequent downstream Smad-dependent signaling cascade activation. The protein is expressed in key developmental organizers including the node, notochord, dorsal somite, and roof plate, where it establishes gradients of BMP activity essential for patterning.
In the adult mouse brain, Noggin is expressed in the subventricular zone (SVZ) where it creates a neurogenic niche by antagonizing endogenous BMPs. This promotes neuronal differentiation of neural stem cells while suppressing astrocytic fate, thereby maintaining neurogenesis throughout adulthood.
生物活性
- Measured by its ability to inhibit BMP-2-induced luciferase reporter activity in HEK293T human embryonic kidney cells. The expected IC50 for this effect is 64-77ng/mL in the presence of 100ng/mL Recombinant Human BMP-2.
Mouse bile duct organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), FGF-10(UA040076), HGF(UA040194) and Wnt Surrogate(UA040250).
Mouse lung organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), FGF-10(UA040076), HGF(UA040194), Wnt Surrogate(UA040250) and KGF/FGF-7(UA040110)
Mouse Gastric organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), Wnt Surrogate(UA040250), KGF/FGF-7(UA040110) and FGF-basic(UA040339)
Mouse small intestinal organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), Wnt Surrogate(UA040250).
Mouse Colon organoids were cultured with R-spondin-1(UA040525), Noggin(UA040404), EGF(UA040126), Wnt Surrogate(UA040250).
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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