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物种
Human分子别名
R-spondin-2,Roof plate-specific spondin-2 (hRspo2)Accession
Q6UXX9表达序列
Gln22-Gly205, with C-terminal Human Fc Tag
表达宿主
HEK293分子量
50-55 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 20ng/ml标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Dev Cell. 2022 Jul 11;57(13):1598-1614.e8.
Int J Mol Sci. 2022 Mar 13;23(6):3089.
RSPO2 (R-spondin 2) is a secreted protein that plays a crucial role in regulating Wnt/β-catenin signaling, a pathway essential for embryonic development, tissue homeostasis, and stem cell maintenance. RSPO2 is highly conserved across species and is known for its ability to potentiate Wnt signaling by interacting with LGR5 and LGR4 receptors.
RSPO2 also plays a role in intestinal organoid cultures. It has been shown to support the growth and differentiation of intestinal stem cells by enhancing Wnt signaling. This is particularly important for maintaining the long-term stability and functionality of intestinal organoids, which are used to study gastrointestinal diseases and develop personalized therapies. In lung organoid cultures, RSPO2 has been shown to enhance the colony-forming efficiency of club cells, a type of progenitor cell in the lung. RSPO2 activates canonical Wnt/β-catenin signaling, which is crucial for the maintenance and expansion of these cells.
生物活性
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The EC50 for this effect is less than 20ng/mL in the presence of 5ng/mL Recombinant Human Wnt Surrogate.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
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