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物种
Human分子别名
Latent TGFB2,MGC116892,TGFβ2,TGF-beta2,Cetermin,Glioblastoma-derived T-cell suppressor factor,G-TSF,表达序列
Leu21-Ser414, with N-terminal His Tag
表达宿主
CHO分子量
12 kDa & 38-55 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 1.0ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 140mM NaCl, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Nature. 1994 Aug 4;370(6488):341-7.
Transforming Growth Factor Beta 2 (TGF-β2) is a member of the TGF-β superfamily, which plays a critical role in various biological processes, including cell proliferation, differentiation, apoptosis, and immune regulation. TGF-β2 is synthesized as a latent complex, consisting of a mature peptide homodimer, a latency-associated peptide (LAP), and latent TGF-β binding proteins (LTBPs). This latent form must be activated to exert its biological effects.
TGF-β2 is involved in multiple physiological and pathological processes. It promotes tissue repair and wound healing but can also contribute to fibrosis and tumor progression. In cancer, TGF-β2 is known to inhibit immune surveillance, facilitating tumor growth and metastasis. Additionally, TGF-β2 signaling is implicated in various diseases, including systemic sclerosis, fibrosis, and Loeys-Dietz syndrome.
生物活性
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The EC50 for this effect is 1ng/mL.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC. Peak which RT=15.9min is mature dimer, while RT=16.2min is LAP dimer.
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