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物种
Mouse分子别名
Transforming growth factor beta-1 proprotein,Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB,Accession
P04202表达序列
Leu30-Ser390
表达宿主
HEK293分子量
13 kDa & 35-55 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 1ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 150mM NaCl, pH7.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Proc Natl Acad Sci U S A. 2023 Nov 28;120(48): e2313228120.
Science. 2018 Nov 23;362(6417):952-956.
Transforming growth factor-beta 1 (TGF-β1) is a critical cytokine that plays a pivotal role in a wide range of biological processes, including cell growth, differentiation, migration, apoptosis, and immune regulation. In mice, TGF-β1 is predominantly secreted in a latent form, which requires activation to exert its biological effects. The latent form of TGF-β1 (L-TGF-β1) is composed of the mature TGF-β1 molecule, the latency-associated peptide (LAP), and the latent TGF-β1-binding protein (LTBP). This complex structure ensures that TGF-β1 is stored in the extracellular matrix (ECM) and can be activated in response to specific cellular and mechanical cues.
The mechanical activation of latent TGF-β1 is a critical mechanism by which cells can modulate TGF-β1 signaling. Forces exerted on the latency-associated peptide (LAP) through integrins can lead to conformational changes in the latent complex, resulting in the release of active TGF-β1. This process is more efficient under conditions of higher cell forces and ECM stiffening, highlighting the importance of mechanical cues in TGF-β1 activation
生物活性
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The EC50 for this effect is less than 1ng/mL after acid activation.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC. Peak which RT=15.1min is mature dimer, while RT=16.6min is LAP dimer.
ELISA
Immobilized Latent TGF-β1 Protein, Mouse (Cat. No. UA040386) at 2.0μg/mL (100μL/well) can bind Biotinylated TGFBR2/TGF-beta RII Fc&Avi Tag Protein, Mouse (Cat. No. UA010847) with EC50 of 41.06-72.68ng/mL.
Immobilized Latent TGF-β1 Protein, Mouse (Cat. No. UA040386) at 2.0μg/mL (100μL/well) can bind TGFBR2 Fc Chimera Protein, Mouse (Cat. No. UA010646) with EC50 of 4.77-6.13ng/mL.
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