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物种
Mouse分子别名
Dickkopf-related protein 1; Dickkopf-1; Dkk-1; mDkk-1;Accession
O54908表达序列
Ser30-His272, with N-terminal His Tag
表达宿主
HEK293分子量
38-70 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 90ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
N Engl J Med. 2003 Dec 25;349(26):2483-94.
Bone. 2006 Oct;39(4):754-66. Epub 2006 May 26.
Dickkopf-1 (DKK-1) is a secreted protein that belongs to the Dickkopf family of Wnt signaling inhibitors. In mice, DKK-1 is expressed in various tissues, including bone, brain, and reproductive organs, and plays a critical role in regulating the Wnt signaling pathway. DKK-1 functions by binding to the Wnt co-receptor LRP5/6, thereby inhibiting the canonical Wnt signaling cascade. By antagonizing Wnt signaling, DKK-1 suppresses the differentiation and proliferation of osteoblasts, leading to the inhibition of bone formation. DKK-1 is also involved in other biological processes, such as embryonic development, tissue homeostasis, and cancer progression. Dysregulation of DKK-1 has been implicated in skeletal disorders, such as osteoporosis and osteoarthritis, as well as in various cancers, highlighting the importance of DKK-1 in development and disease.
生物活性
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293T human embryonic kidney cells. Recombinant Mouse DKK-1 inhibits a constant dose of 30 ng/ml of Recombinant Human Wnt‑Surrogate. The EC50 for this effect is less than 40 ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
引用文献(1)
- Real-time diagnosis of cholangiocarcinoma by combining digestive endoscopy and optic fiber biosensors based on bile clusterin
Long Gao; Baoquan Xiao; Yanyan Lin; Xiao-jia Fang; Shun He; et al
Med. 2025 Sep 11 .
货号：UA040368产品名称：DKK-1 Protein, Mouse
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