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Klotho β Protein, Mouse

Beta-klotho,BKL,Beta Klotho,Klotho beta-like protein

价格 ￥1,000.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040364
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    Beta-klotho; BKL; Beta Klotho; Klotho beta-like protein;

  • Accession

    Q99N32

  • 表达序列

    Phe53-Leu995, with C-terminal His Tag

  • 表达宿主

    HEK293

  • 分子量

    120-140 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE and HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 1.0μg/ml

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose, 5% mannitol

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19796-801. Epub 2007 Dec 3.

背景介绍

  • Klothoβ is a membrane-bound protein that belongs to the Klotho family of proteins, which also includes the soluble form αKlotho. In mice, Klothoβ is predominantly expressed in the kidney, brain, and reproductive organs, and plays a key role in regulating various physiological processes, including aging, mineral metabolism, and insulin signaling. Klothoβ functions as a co-receptor for fibroblast growth factor (FGF) receptors, enhancing the binding of FGF ligands to their receptors and potentiating downstream signaling cascades. Through its interaction with FGF receptors, Klothoβ modulates cell proliferation, differentiation, and survival, as well as metabolic homeostasis and tissue repair. Klothoβ is also involved in the regulation of phosphate and calcium metabolism, and has been implicated in the pathogenesis of age-related diseases and metabolic disorders.

  • 生物活性

    • Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The EC50 for this effect is 1μg/mL in the presence of Recombinant human FGF21.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured Klotho β Protein, Mouse (Cat. No. UA040364), can bind FGF-21 Protein, Rat (Cat. No. UA040231) with an affinity constant of 38.48nM as determined in SPR assay.

    • Anti-His antibody Immobilized on CM5 Chip captured Klotho β Protein, Mouse (Cat. No. UA040364), can bind FGF-21 Protein, , Mouse (Cat. No. UA040070) with an affinity constant of 33.01nM as determined in SPR assay.

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